n–Hexane (C₆H₁₄, CAS No. 110-54-3) is a straight-chain hydrocarbon widely used in HPLC and analytical applications for its purity, volatility, and hydrophobic nature. Often confused with glucose (c 6 h 12 o6), it is distinctly non-polar and ideal for solvent systems. Refined for minimal UV absorbance and high inertness, n-Hexane for HPLC ensures precise, consistent results in pharmaceutical, environmental, food, and chemical labs, making it a trusted choice for high-resolution chromatographic analysis.
n-Hexane offers several distinct chemical properties that make it suitable for analytical-grade applications:
These characteristics support n-Hexane’s widespread use in HPLC, where solvent consistency and purity are crucial for accurate sample analysis. When evaluating solvents, technicians often compare the n hexane formula with other hydrocarbons to determine suitability for specific analytical workflows.
In certain reversed-phase methods, Acetonitrile chemical is used alongside n-hexane to adjust polarity and achieve sharper separation profiles, especially when working with compounds of varying solubility.
Understanding the molecular structure and composition of n-Hexane explains its behaviour as a non-polar solvent:
Its linear configuration and non-polar nature, supported by the n-hexane formula C₆H₁₄, allow n-hexane to dissolve other non-polar compounds efficiently, a critical factor in sample preparation and solvent partitioning in HPLC analysis.
The physical properties of n-Hexane support its performance in high-precision analytical workflows:
| Attribute | Specification |
| Appearance | Clear, colourless liquid |
| Odor | Slight petroleum-like odour |
| Density | Approximately 0.66 g/cm³ at 20°C |
| Evaporation Rate | Rapid evaporation leaves minimal residue |
| Packaging | Amber glass bottles, aluminium cans, HDPE containers, and bulk packaging options |
| Handling Notes | Highly flammable; store in cool, well-ventilated spaces away from ignition sources. Use PPE such as chemical-resistant gloves and safety eyewear. |
Its volatility and purity levels are monitored continuously to ensure stability during transport and storage.
n-Hexane (CAS No. 110-54-3) is generally obtained from crude oil via fractional distillation, followed by advanced purification steps to meet the stringent purity requirements of HPLC-grade solvents.
Manufacturing Steps Include:
The final product is validated against HPLC suitability parameters, including UV absorbance, residue on evaporation, and water content, ensuring compatibility with advanced analytical systems. Quality teams also reference identifiers like 110-54-3 to confirm grade and compliance.
Thanks to its low reactivity and non-polarity, n-Hexane finds use in various high-precision environments. Its behaviour differs significantly from polar compounds such as c 6 h 12 o6, making it suitable for non-polar analytical workflows.
Analytical Chemistry:
Pharmaceutical Testing:
Environmental Analysis:
Industrial Processes:
In lab cleaning and gradient elution workflows, it is sometimes used alongside 2-Propanol for HPLC for better solvent range and column maintenance.
n-Hexane (C₆H₁₄) is a non-polar, volatile solvent ideal for HPLC and advanced analytical techniques, offering high UV transparency and purity. Used in pharmaceutical and environmental testing, it ensures reliable performance and accurate results. At Lab Chemicals, our HPLC-grade n-Hexane meets stringent chromatographic standards, backed by strict quality control and compliance. Choose Lab Chemicals for consistent, high-quality n-Hexane that matches the precision and safety your analysis demands.
What is n-Hexane used for in HPLC?
n-Hexane is primarily used as a non-polar solvent in HPLC for sample extraction, mobile phase preparation, and separation of hydrophobic compounds due to its high purity and low UV absorbance.
What is the CAS number of n-Hexane for HPLC?
The CAS number of n-Hexane is 110-54-3, which uniquely identifies this compound in chemical databases.
What is the n hexane formula?
The n hexane formula is C₆H₁₄, a straight-chain alkane widely used as a non-polar solvent in HPLC applications.
Can n-Hexane be mixed with other solvents for chromatography?
Yes, n-Hexane is commonly mixed with solvents like acetonitrile or 2-propanol to adjust polarity for better separation in HPLC and other analytical techniques.
Is n-Hexane the same as c 6 h 12 o6?
No. n-Hexane is a non-polar hydrocarbon solvent, while c 6 h 12 o6 represents glucose, a polar sugar molecule. They have completely different structures and uses.
Understanding the molecular structure and composition of n-Hexane explains its behaviour as a non-polar solvent:
Its linear configuration and non-polar nature, supported by the n-hexane formula C₆H₁₄, allow n-hexane to dissolve other non-polar compounds efficiently, a critical factor in sample preparation and solvent partitioning in HPLC analysis.
The physical properties of n-Hexane support its performance in high-precision analytical workflows:
| Attribute | Specification |
| Appearance | Clear, colourless liquid |
| Odor | Slight petroleum-like odour |
| Density | Approximately 0.66 g/cm³ at 20°C |
| Evaporation Rate | Rapid evaporation leaves minimal residue |
| Packaging | Amber glass bottles, aluminium cans, HDPE containers, and bulk packaging options |
| Handling Notes | Highly flammable; store in cool, well-ventilated spaces away from ignition sources. Use PPE such as chemical-resistant gloves and safety eyewear. |
Its volatility and purity levels are monitored continuously to ensure stability during transport and storage.
n-Hexane (CAS No. 110-54-3) is generally obtained from crude oil via fractional distillation, followed by advanced purification steps to meet the stringent purity requirements of HPLC-grade solvents.
Manufacturing Steps Include:
The final product is validated against HPLC suitability parameters, including UV absorbance, residue on evaporation, and water content, ensuring compatibility with advanced analytical systems. Quality teams also reference identifiers like 110-54-3 to confirm grade and compliance.
Thanks to its low reactivity and non-polarity, n-Hexane finds use in various high-precision environments. Its behaviour differs significantly from polar compounds such as c 6 h 12 o6, making it suitable for non-polar analytical workflows.
Analytical Chemistry:
Pharmaceutical Testing:
Environmental Analysis:
Industrial Processes:
In lab cleaning and gradient elution workflows, it is sometimes used alongside 2-Propanol for HPLC for better solvent range and column maintenance.
n-Hexane (C₆H₁₄) is a non-polar, volatile solvent ideal for HPLC and advanced analytical techniques, offering high UV transparency and purity. Used in pharmaceutical and environmental testing, it ensures reliable performance and accurate results. At Lab Chemicals, our HPLC-grade n-Hexane meets stringent chromatographic standards, backed by strict quality control and compliance. Choose Lab Chemicals for consistent, high-quality n-Hexane that matches the precision and safety your analysis demands.
What is n-Hexane used for in HPLC?
n-Hexane is primarily used as a non-polar solvent in HPLC for sample extraction, mobile phase preparation, and separation of hydrophobic compounds due to its high purity and low UV absorbance.
What is the CAS number of n-Hexane for HPLC?
The CAS number of n-Hexane is 110-54-3, which uniquely identifies this compound in chemical databases.
What is the n hexane formula?
The n hexane formula is C₆H₁₄, a straight-chain alkane widely used as a non-polar solvent in HPLC applications.
Can n-Hexane be mixed with other solvents for chromatography?
Yes, n-Hexane is commonly mixed with solvents like acetonitrile or 2-propanol to adjust polarity for better separation in HPLC and other analytical techniques.
Is n-Hexane the same as c 6 h 12 o6?
No. n-Hexane is a non-polar hydrocarbon solvent, while c 6 h 12 o6 represents glucose, a polar sugar molecule. They have completely different structures and uses.
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