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Acetonitrile for chromatogra 2,5 L

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Acetonitrile for chromatogra 2,5 L
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Category
Product Code
61803025001730
HSN Code
29269000
CAS Number
-
Brand Name
Merck Millipore
Packing Size
2.5 Lt

What is Acetonitrile?

Acetonitrile (C₂H₃N) is a clear, volatile, polar aprotic solvent essential for HPLC and LC-MS applications. Known for its low UV absorbance and strong solvation power, acetonitrile HPLC grade ensures fast, accurate chromatographic separations in pharmaceutical, environmental, and food testing labs. Lab Chemicals offers a high-purity 2.5 L variant, securely packed for stability and contamination-free performance—ideal for modern analytical workflows. 

Key Chemical Properties of Acetonitrile HPLC

The following properties make HPLC-grade acetonitrile ideal for sensitive analytical applications: 

  • Boiling Point: ~81.6°C 
  • Melting Point: -45°C 
  • Density: ~0.786 g/cm³ at 20°C 
  • Polarity: Strong polar aprotic solvent, ideal for ion-pairing and reversed-phase chromatography 
  • UV Transparency: Low cutoff (~190 nm), excellent for photometric detection 
  • Miscibility: Fully soluble in water, alcohols, and most organics 
  • Volatility: High, enabling fast drying and sample recovery 
  • Stability: Chemically stable across wide pH and temperature ranges 

These qualities ensure reliable performance in qualitative and quantitative HPLC. For non-polar analytes, n-Hexane for HPLC may be combined with acetonitrile to enhance elution and resolution. 

Molecular Formula and Composition

Understanding the chemical structure of acetonitrile clarifies its exceptional behaviour in chromatography: 

  • Chemical Formula: C₂H₃N 
  • Molecular Structure: Comprises a methyl group (–CH₃) connected to a nitrile group (–C≡N). 
  • Molar Mass: 41.05 g/mol 

The linear configuration of the molecule facilitates efficient penetration into stationary phases, improving resolution in high-pressure chromatographic setups. Its minimal interaction with UV detectors enhances signal clarity in trace detection. 

Physical Characteristics – Appearance & Handling

This acetonitrile HPLC grade product is packaged and delivered with a focus on safety and analytical consistency: 

Attribute  Specification 
Appearance  Clear, colourless liquid 
Odor  Mild, ether-like 
Packaging Volume  Supplied in sealed 2.5 L amber-glass bottles with tamper-evident caps 
Evaporation Rate  Fast drying; leaves no residue 
Storage Guidelines  Keep in a cool, dry, and well-ventilated location, away from heat and ignition sources 
Handling Notes  Wear appropriate PPE—gloves, goggles, and lab coats—to prevent inhalation or skin contact 

How is HPLC Grade Acetonitrile Manufactured?

High-purity acetonitrile is produced through a tightly controlled process: 

  • Raw Material Sourcing: Obtained from acrylonitrile byproducts or synthesized from ethylene and ammonia. 
  • Initial Purification: Distilled under reduced pressure to eliminate water and volatiles. 
  • Fine Filtration: Removes particles that may affect chromatography. 
  • Polishing: Advanced adsorption removes UV-active impurities and trace metals. 
  • Quality Testing: Each batch undergoes Karl Fischer, UV absorbance (210–280 nm), and acidity tests. 
  • Inert Packaging: Bottled under nitrogen/argon to prevent degradation. 

Lab Chemicals ensures ISO-certified production for globally compliant HPLC-grade acetonitrile. 

Industrial & Analytical Applications of Acetonitrile

Thanks to its high solvency and low interference characteristics, C₂H₃N finds diverse applications across industries: 

1. High-Performance Liquid Chromatography (HPLC) 

  • Primary eluent in reversed-phase HPLC due to low viscosity and strong elution power. 
  • Enables trace-level detection of pharmaceuticals, pesticides, and biomolecules. 
  • Enhances resolution and peak sharpness in gradient and isocratic runs. 

2. Liquid Chromatography–Mass Spectrometry (LC-MS) 

  • Compatible with electrospray ionization (ESI) interfaces 
  • Provides a clean matrix with low background noise 
  • Used in metabolomics, proteomics, and forensic toxicology 

3. Pharmaceutical Quality Control 

  • Solvent in impurity profiling, assay development, and stability studies 
  • Used in bioequivalence and pharmacokinetic testing 

4. Environmental & Food Testing 

  • Extractant for pesticide and contaminant analysis in soil, water, and food matrices 
  • Enables monitoring of endocrine-disrupting compounds, nitrates, and nitrites 

5. Industrial Processing 

  • Applied in the manufacturing of synthetic fibres, batteries, and photochemical products in non-HPLC grade forms. 
  • Employed in the fabrication of microelectronics and medical devices 

Conclusion

Acetonitrile (C₂H₃N) is a highly versatile solvent prized for its polarity, low viscosity, and UV transparency—making it indispensable in HPLC and advanced chromatography. From pharmaceutical testing to environmental analysis, its reliability spans diverse applications. Lab Chemicals provides HPLC-grade acetonitrile in a 2.5 L variant, ensuring consistent quality and compliance with strict analytical standards. Backed by expert manufacturing and documentation, it's the trusted choice for precision-driven labs. 

Choose Lab Chemicals—where chromatography begins with confidence. 

Molecular Formula and Composition

Understanding the chemical structure of acetonitrile clarifies its exceptional behaviour in chromatography: 

  • Chemical Formula: C₂H₃N 
  • Molecular Structure: Comprises a methyl group (–CH₃) connected to a nitrile group (–C≡N). 
  • Molar Mass: 41.05 g/mol 

The linear configuration of the molecule facilitates efficient penetration into stationary phases, improving resolution in high-pressure chromatographic setups. Its minimal interaction with UV detectors enhances signal clarity in trace detection. 

Physical Characteristics – Appearance & Handling

This acetonitrile HPLC grade product is packaged and delivered with a focus on safety and analytical consistency: 

Attribute  Specification 
Appearance  Clear, colourless liquid 
Odor  Mild, ether-like 
Packaging Volume  Supplied in sealed 2.5 L amber-glass bottles with tamper-evident caps 
Evaporation Rate  Fast drying; leaves no residue 
Storage Guidelines  Keep in a cool, dry, and well-ventilated location, away from heat and ignition sources 
Handling Notes  Wear appropriate PPE—gloves, goggles, and lab coats—to prevent inhalation or skin contact 

How is HPLC Grade Acetonitrile Manufactured?

High-purity acetonitrile is produced through a tightly controlled process: 

  • Raw Material Sourcing: Obtained from acrylonitrile byproducts or synthesized from ethylene and ammonia. 
  • Initial Purification: Distilled under reduced pressure to eliminate water and volatiles. 
  • Fine Filtration: Removes particles that may affect chromatography. 
  • Polishing: Advanced adsorption removes UV-active impurities and trace metals. 
  • Quality Testing: Each batch undergoes Karl Fischer, UV absorbance (210–280 nm), and acidity tests. 
  • Inert Packaging: Bottled under nitrogen/argon to prevent degradation. 

Lab Chemicals ensures ISO-certified production for globally compliant HPLC-grade acetonitrile. 

Industrial & Analytical Applications of Acetonitrile

Thanks to its high solvency and low interference characteristics, C₂H₃N finds diverse applications across industries: 

1. High-Performance Liquid Chromatography (HPLC) 

  • Primary eluent in reversed-phase HPLC due to low viscosity and strong elution power. 
  • Enables trace-level detection of pharmaceuticals, pesticides, and biomolecules. 
  • Enhances resolution and peak sharpness in gradient and isocratic runs. 

2. Liquid Chromatography–Mass Spectrometry (LC-MS) 

  • Compatible with electrospray ionization (ESI) interfaces 
  • Provides a clean matrix with low background noise 
  • Used in metabolomics, proteomics, and forensic toxicology 

3. Pharmaceutical Quality Control 

  • Solvent in impurity profiling, assay development, and stability studies 
  • Used in bioequivalence and pharmacokinetic testing 

4. Environmental & Food Testing 

  • Extractant for pesticide and contaminant analysis in soil, water, and food matrices 
  • Enables monitoring of endocrine-disrupting compounds, nitrates, and nitrites 

5. Industrial Processing 

  • Applied in the manufacturing of synthetic fibres, batteries, and photochemical products in non-HPLC grade forms. 
  • Employed in the fabrication of microelectronics and medical devices 

Conclusion

Acetonitrile (C₂H₃N) is a highly versatile solvent prized for its polarity, low viscosity, and UV transparency—making it indispensable in HPLC and advanced chromatography. From pharmaceutical testing to environmental analysis, its reliability spans diverse applications. Lab Chemicals provides HPLC-grade acetonitrile in a 2.5 L variant, ensuring consistent quality and compliance with strict analytical standards. Backed by expert manufacturing and documentation, it's the trusted choice for precision-driven labs. 

Choose Lab Chemicals—where chromatography begins with confidence. 

Welcome to Jignesh Agency Pvt Ltd, formerly known as Jignesh Agency, established in 1990 by our visionary Director, Jignesh U Pathak. With over three decades of experience, we have emerged as market leaders in India, specializing in providing a comprehensive range of laboratory chemicals, filtration products, bulk solvents, standards, impurities, chromatography consumables, plastic ware, and scientific instruments.

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